r3d100010648 Expressed Sequence Tags database NCBI EST dbEST https://www.ncbi.nlm.nih.gov/genbank/dbest/ FAIRsharing_doi:10.25504/FAIRsharing.v9fya8 MIR:00000159 RRID:SCR_016578 dbEST is a division of GenBank that contains sequence data and other information on "single-pass" cDNA sequences, or "Expressed Sequence Tags", from a number of organisms. Expressed Sequence Tags (ESTs) are short (usually about 300-500 bp), single-pass sequence reads from mRNA (cDNA). Typically they are produced in large batches. They represent a snapshot of genes expressed in a given tissue and/or at a given developmental stage. They are tags (some coding, others not) of expression for a given cDNA library. Most EST projects develop large numbers of sequences. These are commonly submitted to GenBank and dbEST as batches of dozens to thousands of entries, with a great deal of redundancy in the citation, submitter and library information. To improve the efficiency of the submission process for this type of data, we have designed a special streamlined submission process and data format. dbEST also includes sequences that are longer than the traditional ESTs, or are produced as single sequences or in small batches. Among these sequences are products of differential display experiments and RACE experiments. The thing that these sequences have in common with traditional ESTs, regardless of length, quality, or quantity, is that there is little information that can be annotated in the record. If a sequence is later characterized and annotated with biological features such as a coding region, 5'UTR, or 3'UTR, it should be submitted through the regular GenBank submissions procedure (via BankIt or Sequin), even if part of the sequence is already in dbEST. dbEST is reserved for single-pass reads. Assembled sequences should not be submitted to dbEST. GenBank will accept assembled EST submissions for the forthcoming TSA (Transcriptome Shotgun Assembly) division. The individual reads which make up the assembly should be submitted to dbEST, the Trace archive or the Short Read Archive (SRA) prior to the submission of the assemblies. gb-admin@ncbi.nlm.nih.gov info@ncbi.nlm.nih.gov disciplinary 485.518.083 public entries 1995 eng 2 Life Sciences 201 Basic Biological and Medical Research 20105 General Genetics 20107 Bioinformatics and Theoretical Biology 202 Plant Sciences 20207 Plant Genetics 203 Zoology 20306 Animal Genetics, Cell and Developmental Biology 205 Medicine 20503 Human Genetics 21 Biology 22 Medicine Plain text Scientific and statistical data formats Structured graphics other dataProvider DNA RNA animals fruits genome human microorganisms nucleotide plants National Institutes of Health, National Library of Medicine, National Center for Biotechnology Information NIH, NLM, NCBI USA general technical non-profit https://www.ncbi.nlm.nih.gov/ ROR:02meqm098 RRID:SCR_006472 RRID:nif-0000-00139 batch-sub@ncbi.nlm.nih.gov info@ncbi.nlm.nih.gov NCBI Website and Data Usage Policies and Disclaimers https://www.ncbi.nlm.nih.gov/home/about/policies/ open open Copyrights https://www.copyright.gov/ ODC https://opendatacommons.org/licenses/pddl/1.0/ Public Domain https://www.nlm.nih.gov/copyright.html restricted registration About ESTs - How to submit data https://www.ncbi.nlm.nih.gov/genbank/dbest/ unknown yes ftp://ftp.ncbi.nih.gov/repository/dbEST/ other unknown yes 2014-03-10 2021-12-17