Please follow the tutorial on how to use the I-GeneMatcher. If your video does not play you can download the video from here.
The following tables describe the meaning of the columns in the various data tables produced as output by the I-GeneMatcher.
| Column | Description |
| Rank | Rank of guide 1 as reported in CHOPCHOP |
| Match rank | Rank of guide 2 as reported in CHOPCHOP |
| Distance | Precise distance between the guides with the selected configuration |
| Target sequence | Sequence of guide 1 |
| Genomic location | Position of guide 1 |
| Match Genomic location | Position of guide 2 |
| Strand | DNA strand that the guide 1 targets |
| Match Strand | DNA strand that the guide 2 targets |
| GC content (%) | GC content percentage of guide 1 |
| Match GC content (%) | GC content percentage of guide 2 |
| Self-complementarity | Self-complementarity value of guide 1 as reported in CHOPCHOP |
| Match Self-complementarity | Self-complementarity value of guide 2 as reported in CHOPCHOP |
| MM0-3 | Targets containing 0 to 3 mismatches for guide 1 |
| Match MM0-3 | Targets containing 0 to 3 mismatches for guide 2 |
| Efficiency | Efficiency value of guide 1 as reported in CHOPCHOP |
| Match Efficiency | Efficiency value of guide 2 as reported in CHOPCHOP |
| Column | Description | ||||||||
| Rank | Rank of guide 1 as reported in crisprscan | ||||||||
| Match rank | Rank of guide 2 as reported in crisprscan | ||||||||
| Distance | Precise distance between the guides with the selected configuration | ||||||||
| Name | Targeted chromosome of guide 1 | ||||||||
| Match Name | Targeted chromosome of guide 2 | ||||||||
| Start | Starting genomic position of guide 1 | ||||||||
| Match Start | Starting genomic position of guide 2 | ||||||||
| End | Ending genomic position of guide 1 | ||||||||
| Match End | Ending genomic position of guide 2 | ||||||||
| Strand | DNA strand that the guide 1 targets | ||||||||
| Match Strand | DNA strand that the guide 2 targets | ||||||||
| Type | 20 nucleotides following the type of PAM for guide 1 | ||||||||
| Match Type | 20 nucleotides following the type of PAM for guide 2 | ||||||||
| Seq | Targeting sequence containing the PAM sequence of guide 1 | ||||||||
| Match Seq | Targeting sequence containing the PAM sequence of guide 2 | ||||||||
| Sgrna_seq | Sequence of guide 1 | ||||||||
| Match Sgrna_seq | Sequence of guide 2 | ||||||||
| Promoter | If selected during the crisprscan research | ||||||||
| Match Promoter | If selected during the crisprscan research | ||||||||
| Remaining values as reported by crisprscan. Prefix "Match" always indicates the corresponding values of guide 2. | |||||||||
| Column | Description |
| RE sequence | Restriction site of the selected restriction enzyme |
| RE index | Position of the restriction site |
| RE strand | 5'- 3' |
| Distance | Precise distance from the guide |
| Rank | Rank of the guide as reported in CHOPCHOP |
| Target sequence | Sequence of the guide |
| Genomic location | Position of the guide |
| Strand | DNA strand that the guide targets |
| GC content (%) | GC content percentage of the guide |
| Self-complementarity | Self-complementarity value of the guide as reported in CHOPCHOP |
| MM0-3 | Targets containing 0 to 3 mismatches |
| Efficiency | Efficiency value as reported in CHOPCHOP |
| Column | Description | ||||||||
| RE sequence | Restriction site of the selected restriction enzyme | ||||||||
| RE index | Position of the restriction site | ||||||||
| RE strand | 5' - 3' | ||||||||
| Distance | Precise distance from the guide | ||||||||
| Rank | Rank of the guide as reported in crisprscan | ||||||||
| Name | Targeted chromosome | ||||||||
| Start | Starting genomic position of the guide | ||||||||
| End | Ending genomic position of the guide | ||||||||
| Strand | DNA strand that the guide targets | ||||||||
| Type | 20 nucleotides following the type of PAM | ||||||||
| Seq | Targeting sequence containing the PAM sequence | ||||||||
| Sgrna_seq | Sequence of the guide | ||||||||
| Promoter | If selected during the crisprscan research | ||||||||
| Remaining values as reported by crisprscan. | |||||||||