dedup_wf_001::113ca964590682d906a3588d3d6b4838 2020-03-15T05:46:43.509Z 2020-03-15T21:17:13.902Z

oai:pubmedcentral.nih.gov:5657733 oai:doaj.org/article:f26495a4c1d149099049e1a604fa1256 10.1177/0963689717714320 28933215 PMC5657733 10.1177/0963689717714320 Jain, Subhash C. Citron, Bruce A. Vijayalakshmi Ravindranath Saykally, Jessica N. Keeley, Kristen L. Haris Hatic Baglioni, Miriam De Bonis, Michele 2017-06-01 Withania somnifera has been used in traditional medicine for a variety of neural disorders. Recently, chronic neurodegenerative conditions have been shown to benefit from treatment with this extract. To evaluate the action of this extract on traumatically injured neurons, the efficacy of W. somnifera root extract as a neuroprotective agent was examined in cultured model neurons exposed to an in vitro injury system designed to mimic mild traumatic brain injury (TBI). Neuronal health was evaluated by staining with annexin V (an early, apoptotic feature) and monitoring released lactate dehydrogenase activity (a terminal cell loss parameter). Potential mechanisms underlying the observed neuroprotection were examined. Additionally, morphological changes were monitored following injury and treatment. Although no differences were found in the expression of the antioxidant transcription factor nuclear factor erythroid 2-like 2 (Nrf2) or other Nrf2-related downstream components, significant changes were seen in apoptotic signaling. Treatment with the extract resulted in an increased length of neurites projecting from the neuronal cell body after injury. W. somnifera extract treatment also resulted in reduced cell death in the model neuron TBI system. The cell death factor Bax was involved (its expression was reduced 2-fold by the treatment) and injury-induced reduction in neurite lengths and numbers was reversed by the treatment. This all indicates that W. somnifera root extract was neuroprotective and could have therapeutic potential to target factors involved in secondary injury and long-term sequelae of mild TBI. Withania somnifera R Cell Biology neuroprotection SH-SY5Y Biomedical Engineering Transplantation traumatic brain injury neurites Ayurveda Medicine 2018-11-13 2017-6-30 2017-7-1 SAGE Publishing Cell Transplantation, Vol 26 (2017) Cell Transplantation Cell Transplantation true false 0.9 dedup-similarity-result-levenstein wt__________::4de25ac59f6cb729d5716260164bb67c Indian Institute Of Science nih_________::ba7da8316fd53d04a985bc935e438555 INDIAN INSTITUTE OF SCIENCE dedup_wf_001::0047940c0207b6a83e79cd803ecf17d1 MRC - MRC Laboratory of Molecular Biology LMB rcuk________::2558c4f3132f6907f7b23c69009f0d87 INDIAN INSTUTUTE OF SCIENCE dedup_wf_001::d2fdc8e80f8b4365091bcea83f918ccf University of Delhi University of Delhi doiboost____::d5177e3ad00bd9288201b60206a0b5d0 2017-6-30 10.1177/0963689717714320 od_______267::fb470352a4b33af7c83391c02117c4fc SAGE Publications PMC5657733 28933215 2017-06-01 10.1177/0963689717714320 nih_________::24e81ae35bbcb50c778df1039f912617 nih_________::NIH::VETERANS_AFFAIRS 1I01RX001520-01A1 wt__________::52e59d4aa1c57bda1ec144f409de83fc Indian Institute of Science dedup_wf_001::0499ff413ba8e7fa686531725ba12338 IISc Indian Institute of Science wt__________::ba1db3669859a46e72f222052a9a26d8 University of Delhi dedup_wf_001::17c785347dfb060aa115af824b0c6789 IISc Indian Institute of Science Bangalore scholexplore::16181ec1a2484116e8ed6b3348858fe7 28933215 doajarticles::cac994ec6c322070c41474486eb5c595 2017-07-01 SAGE Publishing 10.1177/0963689717714320 r37980778c78::39a72c53d5801325784f728b543a49a1 10.1371/journal.pone.0006628 2016-01-01 Figshare rcuk________::23feba2a5ca7f6b6016bf3a45180da50 University of Delhi true corda_______::30c6b5ab90f30666de1d112fb93d8c77 227878 ec__________::EC::FP7 ec__________::EC::FP7::SP2 ec__________::EC::FP7::SP2::ERC COLLMOT irb_hr______::2330a1d0dac71ffbe15fbcbc807288d4 108-1083570-3635 https://www.ncbi.nlm.nih.gov/pubmed/28933215 2017-06-01 http://europepmc.org/articles/PMC5657733 http://journals.sagepub.com/doi/full-xml/10.1177/0963689717714320 http://journals.sagepub.com/doi/pdf/10.1177/0963689717714320 https://academic.microsoft.com/#/detail/2588640354 2017-07-01 https://doi.org/10.1177/0963689717714320 https://doaj.org/toc/0963-6897 https://doaj.org/toc/1555-3892 http://dx.doi.org/10.1177/0963689717714320 https://journals.sagepub.com/doi/pdf/10.1177/0963689717714320 1 Bryan-Hancock C Harrison J The global burden of traumatic brain injury: preliminary results from the Global Burden of Disease Project. 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