dedup_wf_001::113ca964590682d906a3588d3d6b48382020-03-15T05:46:43.509Z2020-03-15T21:17:13.902Zoai:pubmedcentral.nih.gov:5657733oai:doaj.org/article:f26495a4c1d149099049e1a604fa125610.1177/096368971771432028933215PMC565773310.1177/0963689717714320Extract Protects Model Neurons
from Traumatic InjuryJain, Subhash C.Citron, Bruce A.Vijayalakshmi RavindranathSaykally, Jessica N.Keeley, Kristen L.Haris Hatic2017-06-01Withania somnifera has been used in traditional medicine for a variety
of neural disorders. Recently, chronic neurodegenerative conditions have been
shown to benefit from treatment with this extract. To evaluate the action of
this extract on traumatically injured neurons, the efficacy of W. somnifera root
extract as a neuroprotective agent was examined in cultured model neurons
exposed to an in vitro injury system designed to mimic mild traumatic brain
injury (TBI). Neuronal health was evaluated by staining with annexin V (an
early, apoptotic feature) and monitoring released lactate dehydrogenase activity
(a terminal cell loss parameter). Potential mechanisms underlying the observed
neuroprotection were examined. Additionally, morphological changes were
monitored following injury and treatment. Although no differences were found in
the expression of the antioxidant transcription factor nuclear factor erythroid
2-like 2 (Nrf2) or other Nrf2-related downstream components, significant changes
were seen in apoptotic signaling. Treatment with the extract resulted in an
increased length of neurites projecting from the neuronal cell body after
injury. W. somnifera extract treatment also resulted in reduced cell death in
the model neuron TBI system. The cell death factor Bax was involved (its
expression was reduced 2-fold by the treatment) and injury-induced reduction in
neurite lengths and numbers was reversed by the treatment. This all indicates
that W. somnifera root extract was neuroprotective and could have therapeutic
potential to target factors involved in secondary injury and long-term sequelae
of mild TBI.Withania
somniferaRCell BiologyneuroprotectionSH-SY5YBiomedical EngineeringTransplantationtraumatic
brain injuryneuritesAyurvedaMedicine2018-11-132017-6-302017-7-1SAGE PublishingCell Transplantationtruefalse0.9dedup-similarity-result-levensteinwt__________::4de25ac59f6cb729d5716260164bb67cIndian Institute Of Sciencenih_________::ba7da8316fd53d04a985bc935e438555INDIAN INSTITUTE OF SCIENCEdedup_wf_001::0047940c0207b6a83e79cd803ecf17d1MRC - MRC Laboratory of Molecular BiologyLMBrcuk________::2558c4f3132f6907f7b23c69009f0d87INDIAN INSTUTUTE OF SCIENCEdedup_wf_001::d2fdc8e80f8b4365091bcea83f918ccfUniversity of DelhiUniversity of Delhidoiboost____::d5177e3ad00bd9288201b60206a0b5d02017-6-3010.1177/0963689717714320od_______267::fb470352a4b33af7c83391c02117c4fcSAGE PublicationsPMC5657733289332152017-06-0110.1177/0963689717714320nih_________::24e81ae35bbcb50c778df1039f912617nih_________::NIH::VETERANS_AFFAIRSPreventing TBI-Induced Chronic Functional Loss with a Neuroprotective
Antioxidant1I01RX001520-01A1wt__________::52e59d4aa1c57bda1ec144f409de83fcIndian Institute of Sciencededup_wf_001::0499ff413ba8e7fa686531725ba12338IIScIndian Institute of Sciencewt__________::ba1db3669859a46e72f222052a9a26d8University of Delhidedup_wf_001::17c785347dfb060aa115af824b0c6789IIScIndian Institute of Science Bangalorescholexplore::16181ec1a2484116e8ed6b3348858fe728933215doajarticles::cac994ec6c322070c41474486eb5c5952017-07-01SAGE Publishing10.1177/0963689717714320r37980778c78::39a72c53d5801325784f728b543a49a110.1371/journal.pone.00066282016-01-01Figsharercuk________::23feba2a5ca7f6b6016bf3a45180da50University of Delhihttps://www.ncbi.nlm.nih.gov/pubmed/289332152017-06-01http://europepmc.org/articles/PMC5657733http://journals.sagepub.com/doi/full-xml/10.1177/0963689717714320http://journals.sagepub.com/doi/pdf/10.1177/0963689717714320https://academic.microsoft.com/#/detail/25886403542017-07-01https://doi.org/10.1177/0963689717714320https://doaj.org/toc/0963-6897https://doaj.org/toc/1555-3892http://dx.doi.org/10.1177/0963689717714320https://journals.sagepub.com/doi/pdf/10.1177/09636897177143201 Bryan-Hancock C Harrison J The global burden of traumatic brain
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