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The authors draw from the celebrity politics\u2019 typologies of Paul \u2018t Hart and Karen Tindall, and John Street. Employing a content analysis of newspaper articles from the journals MF Dnes and Blesk during the periods preceding the elections of the Chamber of Deputies, the authors shed light on celebrity involvement in Czech politics. Specifically, they trace three categories mentioned in the media: the celebrity expression of political topics in general, celebrity political endorsement and the candidacy of celebrities. 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Culture experiments can quantify the relationship between planktonic foraminiferal ecology and environmental parameters, but experiments controlling DO have yet to be conducted because it is difficult to maintain a stable oxygen concentration. In this study, we cultured two planktonic foraminifer species ( Orbulina universa  and  Globigerina bulloides ) at six different DO levels between 10% and 100% saturation. Both species showed a high tolerance to low DO, suggesting that \u201cdysoxic\u201d conditions (>\u00a00.7\u00a0mg\u00a0O 2 \u00a0l \u2212\u00a01 ) were not a direct cause of planktonic foraminiferal extinction at OAEs. The high tolerance of these species to extremely low DO might be attributable to their evolutionary descent from benthic foraminifera. Final shell weight increased with increasing DO, suggesting that fossil foraminiferal shell weight could vary with past DO conditions. 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We cloned the galanin gene and analyzed its expression by using in situ hybridization, PCR, and immunocytochemistry throughout the early development of zebrafish until the end of the first week of life. The single zebrafish galanin gene encoded for a single amidated galanin peptide and a galanin message\u2010associated peptide. Two forms resulting from alternative processing were identified. Galanin mRNA was maternally expressed and found in developing fish throughout early development. In situ hybridization showed the first positive neurons in three groups in the brain at 28 hours postfertilization. At 2 days postfertilization, three prosencephalic neuron groups were seen in the preoptic area and in rostral and caudal periventricular hypothalamus. In addition, two other groups of weakly stained neurons were visible, one in the midbrain and another in the hindbrain. Translation inhibition of galanin mRNA with morpholino oligonucleotides caused complete disappearance of galanin immunoreactivity in the brain until 7 dpf and did not induce known cascades of nonspecific pathways or morphological abnormalities. A minor disturbance of sensory ganglia was found. Galanin knockdown did not alter the expression of tyrosine hydroxylases 1 and 2, choline acetyltransferase, histidine decarboxylase, or orexin mRNA. The results suggest that galanin does not regulate the development of these key markers of specific neurons, although galanin\u2010expressing fibers were in a close spatial proximity to several neurons of these neuronal populations. J. Comp. 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The levels of dopamine and noradrenaline decreased significantly after the injection of MPTP and 6\u2010OHDA. Corresponding to these changes, fish exhibited characteristic changes in locomotor behaviour, i.e. the total distance moved and velocity decreased after both neurotoxins. Tyrosine hydroxylase and caspase 3 protein levels were not altered after MPTP or 6\u2010OHDA injections, as studied by immunohistochemistry and western blotting. The catecholaminergic cell clusters suggested to correspond to the mammalian nigrostriatal cell group displayed normal tyrosine hydroxylase immunoreactivity after the toxin treatment and did not show signs of DNA fragmentation that would indicate activation of cascades that lead to cell death. The results show that single systemic injections of MPTP and 6\u2010OHDA induce both biochemical and behavioural changes in zebrafish, albeit failing to produce any significant morphological alteration in catecholaminergic cell clusters at the tested doses. This approach may be used for the screening of chemicals affecting the dopaminergic system. 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Imbalances in the composition of this bacterial community can lead to transient intestinal dysfunctions and chronic disease states. Understanding how to manipulate this ecosystem is thus essential for treating many disorders. In this study, we took advantage of recently developed tools for deep sequencing and phylogenetic clustering to examine the long-term effects of exogenous microbiota transplantation combined with and without an antibiotic pretreatment. In our rat model, deep sequencing revealed an intestinal bacterial diversity exceeding that of the human gut by a factor of two to three. The transplantation produced a marked increase in the microbial diversity of the recipients, which stemmed from both capture of new phylotypes and increase in abundance of others. However, when transplantation was performed after antibiotic intake, the resulting state simply combined the reshaping effects of the individual treatments (including the reduced diversity from antibiotic treatment alone). Therefore, lowering the recipient bacterial load by antibiotic intake prior to transplantation did not increase establishment of the donor phylotypes, although some dominant lineages still transferred successfully. Remarkably, all of these effects were observed after 1 mo of treatment and persisted after 3 mo. Overall, our results indicate that the indigenous gut microbial composition is more plastic that previously anticipated. However, since antibiotic pretreatment counterintuitively interferes with the establishment of an exogenous community, such plasticity is likely conditioned more by the altered microbiome gut homeostasis caused by antibiotics than by the primary bacterial loss. This work was supported in part by grant SAF 2007-64411 (Ministerio de Ciencia e Innovacion, Spain), and by the National Institutes of Health, and the Howard Hughes Medical Institute. 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"inferenceprovenance": "", "invisible": false, "trust": "0.9"}, "value": "A major challenge of biology is understanding the relationship between molecular genetic variation and variation in quantitative traits, including fitness. This relationship determines our ability to predict phenotypes from genotypes and to understand how evolutionary forces shape variation within and between species. Previous efforts to dissect the genotype-phenotype map were based on incomplete genotypic information. Here, we describe the Drosophila melanogaster Genetic Reference Panel (DGRP), a community resource for analysis of population genomics and quantitative traits. The DGRP consists of fully sequenced inbred lines derived from a natural population. Population genomic analyses reveal reduced polymorphism in centromeric autosomal regions and the X chromosome, evidence for positive and negative selection, and rapid evolution of the X chromosome. Many variants in novel genes, most at low frequency, are associated with quantitative traits and explain a large fraction of the phenotypic variance. 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Yamamoto, A. et al. Neurogenetic networks for startle-induced locomotion in Drosophila melanogaster. Proc. Natl Acad. Sci. USA 105, 12393-12398 (2008).</rawText>\n  </reference>\n  <reference position=\"40\">\n    <rawText>40. Harbison, S. T., Yamamoto, A. H., Fanara, J. J., Norga, K. K. &amp; Mackay, T. F. C. Quantitative trait loci affecting starvation resistance in Drosophila melanogaster. Genetics 166, 1807-1823 (2004).</rawText>\n    <id value=\"doi_________::627da378ab8880fa8740618c12e87c0d\" type=\"openaire\" trustLevel=\"0.61566174\"/>\n  </reference>\n  <reference position=\"41\">\n    <rawText>41. Pospisilik, J. A. et al. Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate. Cell 140, 148-160 (2010).</rawText>\n    <id value=\"core_ac_uk__::1229a764ecf16a8b918df455b1cfd248\" type=\"openaire\" trustLevel=\"0.731426\"/>\n  </reference>\n  <reference position=\"42\">\n    <rawText>42. Bellen, H. J. et al. The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. Genetics 167, 761-781 (2004).</rawText>\n  </reference>\n  <reference position=\"43\">\n    <rawText>43. The ModENCODE Consortium. Identification of functional elements and regulatory circuits by Drosophila modENCODE. Science 330, 1787-1797 (2010).</rawText>\n  </reference>\n  <reference position=\"44\">\n    <rawText>44. Charlesworth, B., Coyne, J. A. &amp; Barton, N. H. The relative rates of evolution of sex chromosomes and autosomes. Am. Nat. 130, 113-146 (1987).</rawText>\n  </reference>\n  <reference position=\"45\">\n    <rawText>45. Sieberts, S. K. &amp; Schadt, E. E. Moving toward a system genetics view of disease. Mamm. 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